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In one example, the T7 RNA polymerase-promoter system is used and is induced from the lac operon in BL21 (DE3)-based strains to circumvent the need to separately purify and supplement T7 RNA polymerase into CFE reactions

[DOI] [PMC free article] [PubMed] [Google Scholar] Toparlak

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Microorganisms are retained through a sterile filter membrane (pore size 0.45m), and the filter membrane is then transferred to a culture medium for cultivation

Urinary 8-isoprostane as a biomarker for oxidative stress

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